A fluorescence-based biochip with an integrated holographic diffractive optical element on its underside is presented. The diffractive element is a thick volume hologram written into a layer of photopolymer recording material. The element acts as a collector of the spatially anisotropic fluorescence light emitted from surface-bound fluorophores and redirects the light to a CCD detector. The holographic lithography setup used to fabricate the diffractive elements is described. The performance of the diffractive elements to enhance the light collection is demonstrated.
MACKO Peter;
WHELAN Maurice;
2010-01-13
SPIE
JRC52521
http://dx.doi.org/10.1117/12.831730,
https://publications.jrc.ec.europa.eu/repository/handle/JRC52521,
10.1117/12.831730,
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