Current challenges in detecting food allergens by shotgun and targeted proteomic approaches: A case study on traces of peanut allergens in baked cookies

There is a need for selective and sensitive methods to detect the presence of food allergens at trace levels in highly processed food products. In this work, a combination of non-targeted and targeted proteomics approaches are used to illustrate the difficulties encountered in the detection of the major peanut allergens Ara h 1, Ara h 2 and Ara h 3 from a representative processed food matrix. Shotgun proteomics was employed for selection of the proteotypic peptides for targeted approaches via selective reaction monitoring. Peanut presence through detection of the proteotypic Ara h 3/4 peptides AHVQVVDSNGNR (m/z 432.5, 3+) and SPDIYNPQAGSLK (m/z 695.4, 2+) was confirmed and the developed method was able to detect peanut presence at trace levels (≥10 μg peanut g−1 matrix) in baked cookies.

PEDRESCHI Romina;  NORGAARD Jorgen;  MAQUET Alain; 

2012-10-09

MDPI AG

JRC67360

2072-6643,   

http://www.mdpi.com/2072-6643/4/2/132,    https://publications.jrc.ec.europa.eu/repository/handle/JRC67360,   

10.3390/nu4020132,