Please use this identifier to cite or link to this item:
|Title:||Iron oxide nanoparticle toxicity testing using high throughput assays and high content imaging|
|Authors:||HARRIS GEORGIA; PALOSAARI TAINA; MAGDOLENOVA ZUSANNA; MENNECOZZI MILENA; GINESTE JEAN-MICHEL; SAAVEDRA LUIS; MILCAMPS ANNE; HUK A; COLLINS ANDREW; DUSINSKA MARIA; WHELAN MAURICE|
|Citation:||NANOTOXICOLOGY vol. 9 no. S1 p. 87-94|
|Type:||Articles in periodicals and books|
|Abstract:||Applying validated in vitro assays to the study of nanoparticle toxicity is a growing trend in nanomaterial risk assessment. Precise characterisation of reference nanomaterials and a well-regulated in vitro testing system are required to determine the physicochemical descriptors which dictate the toxic potential of nanomaterials. The use of high throughput, automated technologies to facilitate the identification and prioritization of nanomaterials which could pose a risk is desirable and developments are underway. Mammalian cells were treated with a range of concentrations of iron oxide nanomaterials manufactured for use in medical diagnostics, using a high throughput platform and high content imaging end-points for cell viability, ROS-induced cytotoxicity and DNA damage (double strand breaks). At the same time, the comet assay was employed to measure single and double strand breaks (SBs) and oxidised bases in DNA. Our results show that for iron oxide nanomaterials, these methods provide a fast way to determine the lowest effective concentration and predict the mechanism of toxicity in vitro.|
|JRC Directorate:||Health, Consumers and Reference Materials|
Files in This Item:
There are no files associated with this item.
Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.