Title: Comparative Testing Report on the Detection and Quantification of GM events in compound feedstuff - Comparative testing round: ILC-EURL-GMFF-CT-02/12
Publisher: Publications Office of the European Union
Publication Year: 2013
JRC N°: JRC86873
ISBN: 978-92-79-35041-2
ISSN: 1831-9424
Other Identifiers: EUR 26417
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC86873
DOI: 10.2788/52321
Type: EUR - Scientific and Technical Research Reports
Abstract: The European Union Reference Laboratory for Genetically Modified Food and Feed (EU-RL GMFF (Regulation (EC) No 1829/2003(1)) that is also mandated as EU-RL by Regulation (EC) 882/2006(2) organised a comparative testing round for National Reference Laboratories (NRLs) nominated under Regulation (EC) No 882/2004(2). Participation was open and free of charge for NRLs nominated under Regulation (EC) No 1981/2006(3), for all members of the European Network of GMO Laboratories (ENGL), and for official control laboratories from the EU and third countries. The EU-RL is accredited under ISO 17043 (‘General requirements for proficiency testing’(4)) and this comparative testing round met this ISO Standard(4). The test items were produced in-house from dried leaves of MON 88017 (MON-88Ø17-3) and seeds of soybean event 40-3-2 (MON-Ø4Ø32-6) provided by Monsanto, by spiking a compound feedstuff provided by a Belgian NRL. Participants were required to screen two test items (feedstuff Levels 1 and 2) for the presence of maize events Maize MON 88017, MON 89034 and soybean events 356043, 40-3-2 and MON 89788. Any event detected then had to be quantified. Participants could report the results in mass/mass % or copy/copy % and the EU-RL calculated the robust means (R) of Level 1 and 2 test items accordingly. In addition, "target" values () were assigned by the EU-RL on the basis of its homogeneity study(8) for m/m % data. These values were included in the uncertainty budget. The target standard deviation for CT was fixed by the Advisory Board for Comparative Testing at 0.15 (log10 value) for soybean event 40-3-2 and at 0.20 for maize event MON 88017 based on experience from previous CT rounds. This target standard deviation was used to derive z-scores for the participants’ results. Ninety laboratories from 43 countries registered for this CT round of which 82 from 35 countries returned at least qualitative test results. The results of the qualitative evaluation of the GM content indicated that most of the laboratories correctly detected soybean event 40-3-2 and maize event MON 88017 thus resulting in a very good performance overall. The results of the quantitative evaluation of GM content were found to be satisfactory overall for both events, with 94% of the laboratories submitting results in mass/mass % with a z-score, estimated on the basis of the robust mean, lying within the range of -2 to +2. This percentage decreased to 79% for results expressed in copy/copy %. When asked to repeat experimental work, most of the underperforming laboratories obtained satisfactory results. Only ~57% of participants provided information on measurement uncertainty in a complete and consistent manner, it is apparent therefore that despite the overall satisfactory outcome of this CT round, there is still improvement needed in this crucial area.
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